4TOI
Crystal structure of E.coli ribosomal protein S2 in complex with N-terminal domain of S1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 87.280, 87.280, 94.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.790 - 2.300 |
R-factor | 0.1668 |
Rwork | 0.164 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2qbf and 2oce |
RMSD bond length | 0.008 |
RMSD bond angle | 1.090 |
Data reduction software | XDS (0.1.29) |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.790 | 37.790 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.175 | 0.071 | 1.106 |
Rpim | 0.063 | 0.024 | 0.543 |
Total number of observations | 151502 | 3479 | 7560 |
Number of reflections | 18726 | ||
<I/σ(I)> | 7.4 | 21.7 | 1.5 |
Completeness [%] | 99.0 | 98.1 | 91.3 |
Redundancy | 8.1 | 9.5 | 4.5 |
CC(1/2) | 0.993 | 0.995 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295.15 | 0.1 M HEPES-KOH, pH 7.4, 3 mM MgCl2, 7.5% w/v PEG 6000, 3% w/v 2-methyl-pentanediol-2,4, 100 mM KCl |