4TO8

Methicillin-Resistant Staphylococcus Aureus Class IIb Fructose 1,6-Bisphosphate Aldolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2013-11-24
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	76.464, 96.054, 101.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.502 - 2.100
R-factor	0.1787
Rwork	0.176
R-free	0.22820
RMSD bond length	0.009
RMSD bond angle	1.000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.174	2.173
High resolution limit [Å]	2.100	2.100
Rmerge		0.761
Number of reflections	44357
<I/σ(I)>	12.1	2.31
Completeness [%]	99.3	97.5
Redundancy	7.1	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	ammonium citrate