4TO7

N-Terminal domain of C. Reinhardtii SAS-6 homolog bld12p Q93E F145W K146R (NN24)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	C 2 2 21
Unit cell lengths	42.460, 67.360, 104.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.660 - 1.252
R-factor	0.1681
Rwork	0.167
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qoy
RMSD bond length	0.009
RMSD bond angle	1.246
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.100	1.280
High resolution limit [Å]	1.250	1.250
Rmerge		5.916
Number of reflections	41536
<I/σ(I)>	17.42	0.44
Completeness [%]	98.7	84
Redundancy	12.4	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	PEG3350, MES-NaOH