4TO7
N-Terminal domain of C. Reinhardtii SAS-6 homolog bld12p Q93E F145W K146R (NN24)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.460, 67.360, 104.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.660 - 1.252 |
R-factor | 0.1681 |
Rwork | 0.167 |
R-free | 0.18650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qoy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.246 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.100 | 1.280 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 5.916 | |
Number of reflections | 41536 | |
<I/σ(I)> | 17.42 | 0.44 |
Completeness [%] | 98.7 | 84 |
Redundancy | 12.4 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 293 | PEG3350, MES-NaOH |