4TNF

Crystal Structure of Human Transthyretin Lys15Trp Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	42.920, 85.610, 64.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.200 - 1.600
R-factor	0.2005
Rwork	0.200
R-free	0.21280
RMSD bond length	0.010
RMSD bond angle	1.010
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	64.200
High resolution limit [Å]	1.600
Number of reflections	31899
<I/σ(I)>	15.62
Completeness [%]	99.6
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% (W/V) PEG-1000, 0.1M Imidazole, 0.2M Calcium Acetate