4TN9
Crystal structure of Clostridium histolyticum ColG collagenase polycystic kidney disease-like domain at 1.4 Angstrom resolution
Replaces: 3JQUExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-05 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.919 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 24.998, 71.763, 47.894 |
Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
Resolution | 20.360 - 1.400 |
R-factor | 0.1696 |
Rwork | 0.167 |
R-free | 0.21780 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.650 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
Rmerge | 0.068 | 0.040 | 0.358 |
Total number of observations | 99029 | ||
Number of reflections | 30393 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 92.5 | 89.6 | 83.7 |
Redundancy | 3.3 | 3.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 41-49% MPD, 100 mM Bis-Tris (pH 5.5), 0.1-0.3% ammonium acetate |