4TMS

PLASTIC ADAPTATION TOWARD MUTATIONS IN PROTEINS: STRUCTURAL COMPARISON OF THYMIDYLATE SYNTHASES

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	78.300, 78.300, 243.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.300 *
R-factor	0.193
RMSD bond length	0.018
RMSD bond angle	3.600
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.080 *
Rmerge	NaN *
Total number of observations	66941 *
Number of reflections	22275 *
Completeness [%]	90.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	thymidylate synthases	2.5 (mg/ml)
2	1	drop	EDTA	1 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	potassium phosphate	20 (mM)
5	1	drop	ammonium sulfate	1.15 (M)
6	1	reservoir	ammonium sulfate	2.3 (M)
7	1	reservoir	EDTA	0.2 (mM)
8	1	reservoir	dithiothreitol	1 (mM)
9	1	reservoir	potassium phisphate	20 (mM)