4TM9

Crystal Structure of Human Transthyretin Thr119Trp Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	42.710, 85.690, 63.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.920 - 1.700
R-factor	0.1715
Rwork	0.171
R-free	0.18370
RMSD bond length	0.010
RMSD bond angle	1.030
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.920
High resolution limit [Å]	1.700
Number of reflections	26267
<I/σ(I)>	21.52
Completeness [%]	98.7
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% (W/V) PEG-1000, 0.1M Imidazole, 0.2M Calcium Acetate