4TM0

Kutzneria sp. 744 ornithine N-hydroxylase, KtzI-FADred-ox-NADP+-L-orn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	85.029, 156.401, 164.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.158 - 2.740
R-factor	0.188
Rwork	0.186
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s5w
RMSD bond length	0.014
RMSD bond angle	1.518
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.790
High resolution limit [Å]	2.740	2.740
Number of reflections	57847
<I/σ(I)>	21.3	2.4
Completeness [%]	97.9	97.6
Redundancy	4.5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.9-1.15 M potassium thiocyanate, 22-25% PEG 3350, 0.1 M Bis-tris propane pH 8.5