4TLQ

Crystal structure of C-terminal RNA recognition motif of human ELAV type RNA binding protein-3 at 2.5 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 3
Unit cell lengths	118.611, 118.611, 118.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.508 - 2.503
R-factor	0.1791
Rwork	0.177
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljm
RMSD bond length	0.006
RMSD bond angle	1.119
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	19465
<I/σ(I)>	13.4
Completeness [%]	99.1
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.4 M Ammonium Citrate, 0.1 M Sodium Acetate