4TLK
Crystal Structure of Human Transthyretin Ser85Pro/Glu92Pro Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 41.980, 85.240, 63.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.380 - 1.440 |
R-factor | 0.1892 |
Rwork | 0.189 |
R-free | 0.20140 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 63.380 |
High resolution limit [Å] | 1.440 |
Number of reflections | 48638 |
<I/σ(I)> | 13.34 |
Completeness [%] | 99.0 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% (W/V) PEG-1000, 0.1M Imidazole, 0.2M Calcium Acetate |