4TLH

Monoclinic Crystal Structure of EutL from Clostridium Perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	118.440, 66.030, 79.380
Unit cell angles	90.00, 108.27, 90.00

Refinement procedure

Resolution	33.427 - 1.700
R-factor	0.1644
Rwork	0.163
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.150
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1555))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.427		1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.094	0.026	0.993
Rmeas	0.110	0.031	1.159
Total number of observations	235518
Number of reflections	63757	744	4692
<I/σ(I)>	11.02	33.63	1.62
Completeness [%]	99.5	97.1	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	0.1M sodium potassium phosphate, 0.25 sodium chloride, 10% PEG 8000