4TLG
Crystal structure of SEC14-like protein 4 (SEC14L4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.185, 79.495, 82.395 |
| Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
| Resolution | 37.330 - 1.770 |
| R-factor | 0.14118 |
| Rwork | 0.138 |
| R-free | 0.19425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1olm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.286 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.330 |
| High resolution limit [Å] | 1.770 |
| Rmerge | 0.089 |
| Number of reflections | 640884 |
| <I/σ(I)> | 17.2 |
| Completeness [%] | 95.5 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Magnesium Chloride -- 0.1M tris pH 8.5 -- 25%(w/v) PEG 3350 |
