4TL6

Crystal structure of N-terminal domain of KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-12
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	134.823, 81.057, 95.640
Unit cell angles	90.00, 132.87, 90.00

Refinement procedure

Resolution	38.120 - 1.763
R-factor	0.1705
Rwork	0.169
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gbl
RMSD bond length	0.014
RMSD bond angle	1.478
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.762
Number of reflections	74255
<I/σ(I)>	22.3
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	PEG 400 or PEG 8000