4TL1
GCN4-p1 with mutation to 1-Aminocyclohexanecarboxylic acid at residue 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-12-18 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.322, 30.350, 28.019 |
Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
Resolution | 20.480 - 1.800 |
R-factor | 0.1866 |
Rwork | 0.185 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zta |
RMSD bond length | 0.011 |
RMSD bond angle | 1.106 |
Phasing software | CrystalClear |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.480 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.055 | 0.176 |
Number of reflections | 6386 | |
<I/σ(I)> | 12.9 | 3 |
Completeness [%] | 98.1 | |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M sodium citrate tribasic, 0.1 M sodium cacodylate pH 6.5, 30% v/v isopropanol |