4TKZ

Crystal structure of phosphotransferase system component EIIA from Streptococcus agalactiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	173
Detector technology	CCD
Collection date	2012-06-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	52.293, 53.800, 94.887
Unit cell angles	91.14, 90.02, 60.97

Refinement procedure

Resolution	33.780 - 1.800
R-factor	0.2082
Rwork	0.206
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pdo
RMSD bond length	0.006
RMSD bond angle	1.062
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.074	0.243
Number of reflections	80444
<I/σ(I)>	26.6	3.25
Completeness [%]	96.1	94.5
Redundancy	2.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	293	sodium thiocyanate, PEG 3350