4TKS
Native-SAD phasing for human EGFR kinase domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-12 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 2.0701 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 146.161, 146.161, 146.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.063 - 3.202 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.20920 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.900 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 8731 |
| <I/σ(I)> | 50.3 |
| Completeness [%] | 100.0 |
| Redundancy | 254.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M sodium/potassium tartrate, 100 mM MES, pH 7.0 |
