4TKS

Native-SAD phasing for human EGFR kinase domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-12
Detector	ADSC QUANTUM 4r
Wavelength(s)	2.0701
Spacegroup name	I 2 3
Unit cell lengths	146.161, 146.161, 146.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.063 - 3.202
R-factor	0.189
Rwork	0.188
R-free	0.20920
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.494
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.900
High resolution limit [Å]	3.200
Number of reflections	8731
<I/σ(I)>	50.3
Completeness [%]	100.0
Redundancy	254.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0 M sodium/potassium tartrate, 100 mM MES, pH 7.0