4TKO

Structure of the periplasmic adaptor protein EmrA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03836
Spacegroup name	I 41 2 2
Unit cell lengths	81.366, 81.366, 541.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.850
R-factor	0.2098
Rwork	0.208
R-free	0.24790
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.987
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	3.000
High resolution limit [Å]	2.850	2.850
Rmerge		0.476
Number of reflections	22191
<I/σ(I)>	9.7	3.3
Completeness [%]	100.0	100
Redundancy	11.9	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	288	100mM MES pH 6.5, 100mM MgCl2, 10% isopropanol, 8% PEG4000