4TKK
Sulfolobus solfataricus HJC mutants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 103.15 |
Detector technology | CCD |
Collection date | 2000-11-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.86 |
Spacegroup name | I 2 3 |
Unit cell lengths | 141.172, 141.172, 141.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.230 - 2.400 |
R-factor | 0.2043 |
Rwork | 0.202 |
R-free | 0.24760 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 18314 |
<I/σ(I)> | 22.7 |
Completeness [%] | 99.4 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | (26.6% v/v PEG 400, 5% glycerol, 0.19M calcium chloride and 0.095M HEPES pH 7.4 |