4TKH
The 0.93 angstrom X-ray structure of the human heart fatty acid-binding protein complexed with myristic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.80000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.568, 69.475, 33.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.910 - 0.930 |
| R-factor | 0.11575 |
| Rwork | 0.115 |
| R-free | 0.12559 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.311 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.950 |
| High resolution limit [Å] | 0.930 | 0.930 |
| Rmerge | 0.399 | |
| Number of reflections | 86123 | |
| <I/σ(I)> | 10.1 | 3.49 |
| Completeness [%] | 98.9 | 97.9 |
| Redundancy | 11.6 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 55%(v/v) PEG400 |
