4TKD

Sulfolobus solfataricus HJC mutants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	103.15
Detector technology	CCD
Collection date	2002-05-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	64.601, 61.834, 55.245
Unit cell angles	90.00, 95.74, 90.00

Refinement procedure

Resolution	19.930 - 2.010
R-factor	0.1967
Rwork	0.194
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.180
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Number of reflections	21989
<I/σ(I)>	15.2
Completeness [%]	76.2
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10 % (w/v) PEG 6000, 5 % (v/v) PEG 400, and 0.1 M citric acid pH 5.0