4TKC
Japanese Marasmius oreades lectin complexed with mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SAGA-LS BEAMLINE BL07 |
| Synchrotron site | SAGA-LS |
| Beamline | BL07 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-24 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.420, 58.420, 57.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.590 - 1.290 |
| R-factor | 0.16164 |
| Rwork | 0.159 |
| R-free | 0.20688 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.587 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.590 |
| High resolution limit [Å] | 1.290 |
| Number of reflections | 29101 |
| <I/σ(I)> | 7.2 |
| Completeness [%] | 99.9 |
| Redundancy | 10.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.5M Ammonium sulfate, 0.1M Sodium citrate tribasic dihydrate, 1.0M Lithium sulfate monohydrate, 5% 2-propanol, 0.2M mannose |
