4TK5

Crystal Structure of human Tankyrase 2 in complex with EB47.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-07
Detector	MAR CCD 165 mm
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	74.020, 79.620, 153.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.570 - 2.020
R-factor	0.1772
Rwork	0.177
R-free	0.21200
RMSD bond length	0.009
RMSD bond angle	1.050
Data reduction software	XDS (2005/3)
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.620	79.620	2.120
High resolution limit [Å]	2.020	5.650	2.020
Rmerge	0.075	0.038	0.403
Total number of observations	843685
Number of reflections	60172	3011	35
<I/σ(I)>	26.39	61.09	2.15
Completeness [%]	99.5	99.9	96.5
Redundancy	13.95	13.37	8.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M NaCl, 0.1M Sodium, Hepes buffer at pH7.5, 12-15% iso-propanol