4TK4

GephE in complex with a GABA receptor alpha3 subunit derived double mutant peptide in space group P61

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9393
Spacegroup name	P 61
Unit cell lengths	160.180, 160.180, 127.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.880 - 3.601
R-factor	0.1784
Rwork	0.176
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fu3
RMSD bond length	0.003
RMSD bond angle	0.707
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	67.890
High resolution limit [Å]	3.600
Number of reflections	21728
<I/σ(I)>	7.6
Completeness [%]	100.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.15 M Calcium acetate, 0.1 M sodium cacodylate pH 6.0, 6-10 % Isopropanol