4TK3

Geph E in complex with a GABA receptor alpha3 derived double mutant peptide in spacegroup P21212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.8726
Spacegroup name	P 21 21 2
Unit cell lengths	110.027, 157.184, 51.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.533 - 2.700
R-factor	0.2307
Rwork	0.229
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fu3
RMSD bond length	0.003
RMSD bond angle	0.631
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.540
High resolution limit [Å]	2.700
Number of reflections	25090
<I/σ(I)>	9.1
Completeness [%]	100.0
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M Tris pH 7.5, 21-27% PEG 4000