4TK2
Geph E in complex with a GABA receptor alpha3 subunit derived peptide in space group P61
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9393 |
Spacegroup name | P 61 |
Unit cell lengths | 164.510, 164.510, 129.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.900 - 4.100 |
R-factor | 0.186 |
Rwork | 0.183 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fu3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.584 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.720 | |
High resolution limit [Å] | 4.100 | |
Number of reflections | 15728 | |
<I/σ(I)> | 6 | 1.6 |
Completeness [%] | 99.0 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.15 M Calcium acetate, 0.1 M sodium cacodylate pH 6.0, 6-10 % Isopropanol |