4TK1
Geph E in complex with a GABA receptor alpha3 subunit derived peptide in space group P21212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 110.439, 157.695, 51.018 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.540 - 2.700 |
R-factor | 0.21759 |
Rwork | 0.215 |
R-free | 0.26441 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fu3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.293 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.600 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.212 |
Number of reflections | 25198 |
<I/σ(I)> | 7.8 |
Completeness [%] | 99.0 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Tris pH 7.5, 21-27% PEG 4000 |