4TF4
ENDO/EXOCELLULASE:CELLOPENTAOSE FROM THERMOMONOSPORA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-06 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 145.680, 145.680, 157.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 * |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.160 | 0.300 |
| Number of reflections | 101890 | |
| <I/σ(I)> | 5.7 | 2.3 |
| Completeness [%] | 91.0 | 81 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 4 * | 90MG/ML E4-68 26% PEG8000 0.7M LICL 0.1M NA CITRATE, PH5.8 4 DEGREE, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | E4-68 | 90 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 26 (%) | |
| 3 | 1 | reservoir | 0.7 (M) | ||
| 4 | 1 | reservoir | Na-citrate | 0.1 (M) |
