4S37
Crystal structure of R2 pyocin membrane-piercing spike
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.945, 137.471, 164.759 |
| Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
| Resolution | 49.186 - 2.200 |
| R-factor | 0.1648 |
| Rwork | 0.163 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.889 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.260 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.414 |
| Number of reflections | 203160 | |
| <I/σ(I)> | 15.1 | 3.2 |
| Completeness [%] | 98.8 | 95.3 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 60% PEG 400, 100mM NaSO4, 100mM Bis-Tris, pH 8.5, 18mg/ml protein, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
