4S21

Crystal structure of the photosensory core module of bacteriophytochrome RPA3015 from R. palustris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-09
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	175.360, 175.360, 96.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.892 - 3.250
R-factor	0.2642
Rwork	0.262
R-free	0.31560
Structure solution method	SAD
Starting model (for MR)	2ool
RMSD bond length	0.007
RMSD bond angle	1.480
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Rmerge	0.048
Number of reflections	26676
Completeness [%]	99.4
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10mg/ml protein, 1.4M sodium acetate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K