4S1V
Crystal structure of phosphoglycerate oxidoreductase from Vibrio Cholerae o395
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.916, 83.258, 85.986 |
| Unit cell angles | 64.39, 81.94, 76.03 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gg9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 70407 | |
| <I/σ(I)> | 15.86 | 2.69 |
| Completeness [%] | 95.6 | 92.9 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 273 | 10% PEG 3350, 10% ISOPROPANOL (V/V), 0.3M LICL, 0.1M SODIUM CITRATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
