4S1L

Structure of Uranotaenia sapphirina cypovirus (CPV17) polyhedrin at 298 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	298
Detector technology	FLAT PANEL
Collection date	2013-03-16
Detector	Cornell-SLAC Pixel Array Detector (CSPAD)
Wavelength(s)	1.456
Spacegroup name	I 2 3
Unit cell lengths	106.100, 106.100, 106.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 1.752
R-factor	0.1238
Rwork	0.122
R-free	0.15430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.386
Data reduction software	cctbx.xfel
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1737))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.300	28.300
High resolution limit [Å]	1.750	1.750
Number of reflections	20122
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	In vivo	7.5	301	CRYSTALS FORMED NATURALLY WITHIN THE CYTOPLASM AND WERE PURIFIED FROM CELLS, pH 7.5, In vivo, temperature 301K