4S1H
Pyridoxal kinase of Entamoeba histolytica with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.256, 44.272, 75.246 |
| Unit cell angles | 89.94, 102.89, 107.03 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.21894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native Pyridoxal Kinase from Entamoeba histolytica |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 63103 | |
| <I/σ(I)> | 29.34 | 3.67 |
| Completeness [%] | 89.8 | 48.5 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 273 | 15% Peg4000, 100mM Tris pH7.5, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
