4RZC
Fv M6P-1 in complex with mannose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.607, 128.050, 127.295 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.090 - 2.723 |
R-factor | 0.2283 |
Rwork | 0.226 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.489 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.090 | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.800 | 2.700 |
Rmerge | 0.105 | 0.053 | 0.392 |
Number of reflections | 21952 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 81.2 | 77.7 | 86.3 |
Redundancy | 2.5 | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | Tris, Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |