4RZA

Pre-mRNA-splicing factor 38A AS 1-205

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Detector technology	CCD
Collection date	2013-10-31
Detector	RAYONIX MX-225
Wavelength(s)	0.895
Spacegroup name	P 21 21 21
Unit cell lengths	42.612, 59.433, 68.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.980 - 1.900
R-factor	0.1601
Rwork	0.158
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.992
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.980
High resolution limit [Å]	1.900
Number of reflections	14297

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	0.1 M MES pH 5.0, 16% PEG 3350, 0.2 M (NH4)2SO4, and 5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K