4RZA
Pre-mRNA-splicing factor 38A AS 1-205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Detector technology | CCD |
| Collection date | 2013-10-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.612, 59.433, 68.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.980 - 1.900 |
| R-factor | 0.1601 |
| Rwork | 0.158 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.980 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 14297 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1 M MES pH 5.0, 16% PEG 3350, 0.2 M (NH4)2SO4, and 5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
