4RZ9

Pre-mRNA-splicing factor 38A AS 1-179

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Detector technology	CCD
Collection date	2011-09-26
Detector	RAYONIX MX-225
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	41.886, 61.848, 67.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.040 - 1.280
R-factor	0.1276
Rwork	0.126
R-free	0.15020
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.354
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.040
High resolution limit [Å]	1.280
Rmerge	0.055
Number of reflections	46008

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MES pH 6.5, and 15% (w/v) PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K