4RZ4
Fructose-6-phosphate aldolase Q59E Y131F from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.440, 133.000, 102.270 |
| Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
| Resolution | 88.440 - 1.750 |
| R-factor | 0.18003 |
| Rwork | 0.179 |
| R-free | 0.19585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6w pentamer |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.440 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.700 |
| Number of reflections | 236188 | |
| <I/σ(I)> | 9.5 | 1.8 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH 8.5, 0.2M NaCl, 21% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
