4RZ3
Crystal structure of the MinD-like ATPase FlhG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-06-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.250, 72.560, 65.620 |
| Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
| Resolution | 29.840 - 1.900 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rz2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.247 |
| Number of reflections | 37540 | |
| <I/σ(I)> | 12 | 5.8 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES-Na, pH 7.0, 20 % (w/v) PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
