4RZ2

Crystal structure of the MinD-like ATPase FlhG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	77
Detector technology	CCD
Collection date	2011-10-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	63.870, 89.360, 111.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.416 - 2.800
R-factor	0.1824
Rwork	0.177
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ql9
RMSD bond length	0.008
RMSD bond angle	1.228
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.960	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.136	0.470
Number of reflections	16375
<I/σ(I)>	11.7	5.2
Completeness [%]	100.0	100
Redundancy	5.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, pH 7.5, 10 % (w/v) PEG 8000, 0.1 M Urea, VAPOR DIFFUSION, SITTING DROP, temperature 293K