4RYZ
Crystal structure of RPE65 in complex with S-emixustat and palmitate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 65 |
| Unit cell lengths | 175.603, 175.603, 86.323 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.840 - 2.500 |
| R-factor | 0.17906 |
| Rwork | 0.177 |
| R-free | 0.21906 |
| Structure solution method | rigid body refinement |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.117 | 0.915 |
| Number of reflections | 50434 | |
| <I/σ(I)> | 10.11 | 1.1 |
| Completeness [%] | 96.2 | 94.7 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 281 | 40% PEG 300, 200 mM NaCl, 100 mM CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
