4RYK
Crystal structure of a putative transcriptional regulator from Listeria monocytogenes EGD-e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 86.310, 103.930, 112.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.400 - 2.090 |
| R-factor | 0.1694 |
| Rwork | 0.168 |
| R-free | 0.19919 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.400 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.040 | 0.658 |
| Number of reflections | 30178 | |
| <I/σ(I)> | 27.1 | 3 |
| Completeness [%] | 99.4 | 98.1 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.1 M Ammonium Tartrate, 25 % Sucrose, 10 % PEG 400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
