4RY9

Crystal structure of carbohydrate transporter solute binding protein VEIS_2079 from Verminephrobacter eiseniae EF01-2, TARGET EFI-511009, a complex with D-TALITOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-05
Detector	RAYONIX MX225HE
Spacegroup name	P 2 2 21
Unit cell lengths	68.650, 68.635, 120.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.400
R-factor	0.12403
Rwork	0.123
R-free	0.15090
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.687
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.095	0.980
Number of reflections	113208
<I/σ(I)>	33.9	2.8
Completeness [%]	97.5	95.4
Redundancy	15	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		PROTEIN (10 MM HEPES PH 7.5, 5 MM DTT, 10 MM D-TALITOL) RESERVOIR: 0.2 M POTASSIUM SULFATE, 25% PEG3350 CRYOPROTECTION: RESERVOIR SOLUTION PLUS 25% (V/V) GLYCEROL VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K