4RY7
C-terminal mutant (D559E) of HCV/J4 RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.000, 107.650, 133.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.947 - 3.000 |
| R-factor | 0.1787 |
| Rwork | 0.173 |
| R-free | 0.25830 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nb4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 29813 | |
| <I/σ(I)> | 9.3 | 2.8 |
| Completeness [%] | 95.2 | 84.3 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 50 mM MES, pH 5.0, 20% PEG4000, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
