4RY3

Crystal structure of human Fanconi-associated nuclease 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.068
Spacegroup name	P 21 3
Unit cell lengths	161.082, 161.082, 161.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.802
R-factor	0.2097
Rwork	0.208
R-free	0.24330
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.252
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	34401
Completeness [%]	99.5	100
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	277	2.5M sodium acetate, 0.1M Bis-Tris propane, 10% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K