4RY1
Crystal structure of periplasmic solute binding protein ECA2210 from Pectobacterium atrosepticum SCRI1043, Target EFI-510858
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-05 |
Detector | RAYONIX MX225HE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.751, 93.669, 99.148 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.400 |
R-factor | 0.13739 |
Rwork | 0.136 |
R-free | 0.17647 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.461 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.104 | 0.690 |
Number of reflections | 160376 | |
<I/σ(I)> | 22.2 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | PROTEIN (10 MM HEPES PH 7.5, 5 MM DTT); RESERVOIR: 0.085 M TRIS-HCL, PH 8.5, 0.17 M SODIUM ACETATE, 25% PEG3350, 15% (V/V) GLYCEROL; CRYOPROTECTION: RESERVOIR SOLUTION, VAPOR DIFFUSION, SITTING DROP, temperature 294K |