4RY0

Crystal structure of ribose transporter solute binding protein RHE_PF00037 from Rhizobium etli CFN 42, TARGET EFI-511357, in complex with D-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-05
Detector	RAYONIX MX225HE
Spacegroup name	C 1 2 1
Unit cell lengths	93.110, 36.766, 95.480
Unit cell angles	90.00, 117.91, 90.00

Refinement procedure

Resolution	24.000 - 1.400
R-factor	0.1446
Rwork	0.143
R-free	0.18106
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.537
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.103	0.800
Number of reflections	57304
<I/σ(I)>	22.2	2.57
Completeness [%]	98.0	96.6
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		PROTEIN (10 MM HEPES PH 7.5, 5 MM DTT, 10 MM D-RIBOSE); RESERVOIR: 0.2 M CALCIUM CHLORIDE, 0.1 M TRIS-HCL, PH 8.5, 20% (w/v) PEG 4000; CRYOPROTECTION: RESERVOIR SOLUTION PLUS 20% D-GLUCOSE SOLUTION; VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K