4RXX

Crystal Structure of the N-terminal Domain of Human Ubiquitin Specific Protease 38

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97932
Spacegroup name	P 21 21 21
Unit cell lengths	41.985, 102.039, 135.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.060
R-factor	0.1906
Rwork	0.189
R-free	0.24190
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.324
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.100
High resolution limit [Å]	2.060	5.590	2.060
Rmerge	0.127	0.076	0.820
Number of reflections	37072
<I/σ(I)>	22.9
Completeness [%]	99.8	100	97.3
Redundancy	9.7	9.4	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Protein sample was first mixed Hampton Research Additive Screen H03 40% in 10:1 (V/V) Protein:1,3-propanediol ratio, then set up with 15% PEG8000, 0.2 M MgCl2, 0.1 M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 291K, VAPOR DIFFUSION, HANGING DROP