4RXV
The crystal structure of the N-terminal fragment of uncharacterized protein from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.515, 68.939, 40.947 |
| Unit cell angles | 90.00, 112.53, 90.00 |
Refinement procedure
| Resolution | 37.821 - 1.099 |
| R-factor | 0.1251 |
| Rwork | 0.124 |
| R-free | 0.13920 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.365 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1834)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.821 | 1.120 |
| High resolution limit [Å] | 1.099 | 1.090 |
| Rmerge | 0.048 | 0.050 |
| Number of reflections | 62764 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 90.1 | 45.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.6 M ammonium sulfate, 0.1 M NaCl, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
