4RXI
Structure of C-terminal domain of uncharacterized protein from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.353, 60.715, 57.524 |
| Unit cell angles | 90.00, 104.61, 90.00 |
Refinement procedure
| Resolution | 38.491 - 1.900 |
| R-factor | 0.1573 |
| Rwork | 0.156 |
| R-free | 0.18320 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1834)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | |
| Number of reflections | 22413 | |
| <I/σ(I)> | 35 | |
| Completeness [%] | 97.9 | 95.4 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.6M NH4Sulfate, 0.1M NaCl, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
