4RXG

Fructose-6-phosphate aldolase Q59E from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-01
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.931
Spacegroup name	P 21 21 21
Unit cell lengths	115.436, 126.748, 183.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.500 - 2.154
R-factor	0.19066
Rwork	0.189
R-free	0.22807
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l6w
RMSD bond length	0.012
RMSD bond angle	1.506
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.500	2.570
High resolution limit [Å]	2.154	2.154
Rmerge	0.120	0.450
Number of reflections	142189
<I/σ(I)>	10	3.4
Completeness [%]	99.8	99.9
Redundancy	5.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1