4RXF
Fructose-6-phosphate aldolase Y131F from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91844 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.400, 133.602, 101.590 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 101.490 - 2.400 |
| R-factor | 0.19595 |
| Rwork | 0.194 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.490 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.105 | 0.465 |
| Number of reflections | 91562 | |
| <I/σ(I)> | 12.7 | 3.7 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.2 | 19% PEG3350, 0.1M HEPES pH 8, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |
